Intern
    Meningokokken und Haemophilus Influenzae

    Data of the Consulting Laboratory for Haemophilus influenzae 2012

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    1. Introduction

     

    Since December, 2007, the Consulting Laboratory for Haemophilus influenzae (KLHi) has been assigned the laboratory surveillance of invasive H. influenzae diseases in Germany by the Robert Koch Institute. Its tasks include serotyping of clinical isolates and the detection of antibiotic resistance against ß-Lactams. In 2012 submissions from 277 patients were analysed.

    In 228 of the submitted cases, H. influenzae was detected from primarily sterile materials (this figure equals the number of invasive diseases). The age group most affected by invasive H. influenzae disease is > 40 years (180 cases, 78.9 %).

    The vast majority of the cases consisted of non-typeable H. influenzae (NTHi, 187 cases, 81.6 %), followed by Hif as the most frequent capsular serotype (26 cases; 11.4 %). Hie showed third highest frequency among the serotypes (8 cases; 3.5 %), closely followed by Hib (6 cases, 2.6 %). In one case, where detection was carried out by molecular methods, the serotype was not determined.

    The KLHi analyzed the frequency of ampicillin resistance of invasive isolates. In 2012, ampicillin E-tests were carried out in the 224 vital isolates submitted to the KLHi (in 4 cases, no viable isolate was available). In 11.6 % (26 isolates) of the clinical isolates a MIC > 1 µg/ml was detected, and18 of these isolates (69.2 %) showed β‑lactamase production.

     

    2. Serotype distribution of German invasive H. influenzae isolates in 2012

     

    3. Age distribution of patients with invasive H. influenzae disease

    4. Serotype distribution in Federal States

     

    BW

    BY

    BE

    BB

    HB

    HH

    HE

    MV

    NI

    NW

    RP

    SL

    SN

    ST

    SH

    TH

    Hib

    1

    1

    0

    0

    0

    0

    1

    0

    1

    1

    0

    0

    0

    0

    1

    0

    Hie

    0

    1

    2

    0

    0

    1

    0

    0

    1

    1

    0

    0

    1

    0

    0

    1

    Hif

    8

    4

    4

    0

    1

    0

    1

    1

    0

    3

    0

    0

    3

    0

    1

    0

    NTHi

    34

    26

    13

    7

    1

    6

    7

    2

    10

    60

    7

    2

    3

    0

    7

    2

    n.d.

    1

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    0

    BW: Baden-Württemberg, BY: Bavaria, BE: Berlin, BB: Brandenburg, HB: Bremen, HH: Hamburg, HE: Hesse, MV: Mecklenburg-Vorpommern, NI: Lower Saxony, NW: North Rhine-Westfalia, RP: Rhineland-Palatinate, SL: Saarland, SN: Saxony, ST: Saxony-Anhalt, SH: Schleswig-Holstein, TH: Thuringia.

    5. Serotype distribution according to site of isolation

     

    Hib

    Hie

    Hif

    NTHi*

    n.d.

    Blood

    3

    5

    23

    179

    0

    Cerebrospinal fluid

    3

    3

    3

    13

    1

     

    * in 5 cases, H. influenzae was detected in both blood and CSF.

     

    6. Ampicillin resistance in invasive H. influenzae isolates

    7. Publications of the KLHi

    Original Articles

    - Lâm TT, Elias J, Frosch M, Vogel U, Claus H. New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142. Int J Med Microbiol. 2011 Feb;301(2):176-9.

    - Lâm TT, Frosch M, Claus H, Vogel U. Sequence analysis of the serotype-specific synthesis regions II of Haemophilus influenzae serotypes c and d: evidence for common ancestry of capsule synthesis in Pasteurellaceae and Neisseria meningitidis. Research in Microbiology 2011 Jun;162(5):483-7.

    Reviews

    - Lâm TT, Claus H, Elias J, Hellenbrand W, Imöhl M, Prelog M, Sing A, van der Linden M, Vogel U. Infections with Pneumococci, Menigococci, H. influenzae and Diphtheria in Germany: the RKI Reference Network for Invasive Bacterial Infections (IBI) at the 5th Würzburg Workshop on Epidemiology, Prevention and Therapy for Invasive Meningococcal Diseases 2010 (Meeting Report). Gesundheitswesen. 2011. DOI: 10.1055/s-0031-1286269.

    Disclaimer: the above data were generated with federal funds (RKI). Scientific use is prohibited without prior written consent by KLHi or RKI. Commercial use is strictly prohibited. Inclusion of figures or tables in talks or oral presentations is not allowed. 

     

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    97080 Würzburg

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